Multianalyte liquid biopsy to aid the diagnostic workup of breast cancer
-
Published:2022-09-27
Issue:1
Volume:8
Page:
-
ISSN:2374-4677
-
Container-title:npj Breast Cancer
-
language:en
-
Short-container-title:npj Breast Cancer
Author:
Setayesh Sonia Maryam, Hart Olivia, Naghdloo Amin, Higa Nikki, Nieva Jorge, Lu Janice, Hwang Shelley, Wilkinson Kathy, Kidd Michael, Anderson AmandaORCID, Velasco Carmen Ruiz, Kolatkar AnandORCID, Matsumoto Nicholas, Nevarez Rafael, Hicks James B.ORCID, Mason JeremyORCID, Shishido Stephanie N., Kuhn PeterORCID
Abstract
AbstractBreast cancer (BC) affects 1 in every 8 women in the United States and is currently the most prevalent cancer worldwide. Precise staging at diagnosis and prognosis are essential components for the clinical management of BC patients. In this study, we set out to evaluate the feasibility of the high-definition single cell (HDSCA) liquid biopsy (LBx) platform to stratify late-stage BC, early-stage BC, and normal donors using peripheral blood samples. Utilizing 5 biomarkers, we identified rare circulating events with epithelial, mesenchymal, endothelial and hematological origin. We detected a higher level of CTCs in late-stage patients, compared to the early-stage and normal donors. Additionally, we observed more tumor-associated large extracellular vesicles (LEVs) in the early-stage, compared to late-stage and the normal donor groups. Overall, we were able to detect reproducible patterns in the enumeration of rare cells and LEVs of cancer vs. normal donors and early-stage vs. late-stage BC with high accuracy, allowing for robust stratification. Our findings illustrate the feasibility of the LBx assay to provide robust detection of rare circulating events in peripheral blood draws and to stratify late-stage BC, early-stage BC, and normal donor samples.
Funder
Breast Cancer Research Foundation U.S. Department of Health & Human Services | NIH | National Cancer Institute Winnie and James Hart Endowed Fellowship, 2021 USC Dornsife Student Opportunities for Academic Research Fellowship, 2021 Kalayil and Leela Chacko, M.D. Fellowship
Publisher
Springer Science and Business Media LLC
Subject
Pharmacology (medical),Radiology, Nuclear Medicine and imaging,Oncology
Reference73 articles.
1. Siegel, R. L., Miller, K. D., Fuchs, H. E. & Jemal, A. Cancer statistics, 2021. CA Cancer J. Clin. 71, 7–33 (2021). 2. American Cancer Society, Cancer Facts & Figures 2021, Atlanta: American Cancer Society, (2021). 3. American Cancer Society. Breast Cancer Facts & Figures 2019-2020, Atlanta: American Cancer Society, Inc., (2019). 4. Amin MB et al. Cancer Staging Manual, AJCC (American Joint Committee on Cancer), 8th edition, 3rd printing, (2018). 5. Mariotto, A. B., Etzioni, R., Hurlbert, M., Penberthy, L. & Mayer, M. Estimation of the Number of Women Living with Metastatic Breast Cancer in the United States. Cancer Epidemiol. Biomark. Prev. 26, 809–815 (2017).
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|