Abstract
AbstractMining is among the human activities with widest environmental impacts, and mining-impacted environments are characterized by high levels of metals that can co-select for antibiotic resistance genes (ARGs) in microorganisms. However, ARGs in mining-impacted environments are still poorly understood. Here, we conducted a comprehensive study of ARGs in such environments worldwide, taking advantage of 272 metagenomes generated from a global-scale data collection and two national sampling efforts in China. The average total abundance of the ARGs in globally distributed studied mine sites was 1572 times per gigabase, being rivaling that of urban sewage but much higher than that of freshwater sediments. Multidrug resistance genes accounted for 40% of the total ARG abundance, tended to co-occur with multimetal resistance genes, and were highly mobile (e.g. on average 16% occurring on plasmids). Among the 1848 high-quality metagenome-assembled genomes (MAGs), 85% carried at least one multidrug resistance gene plus one multimetal resistance gene. These high-quality ARG-carrying MAGs considerably expanded the phylogenetic diversity of ARG hosts, providing the first representatives of ARG-carrying MAGs for the Archaea domain and three bacterial phyla. Moreover, 54 high-quality ARG-carrying MAGs were identified as potential pathogens. Our findings suggest that mining-impacted environments worldwide are underexplored hotspots of multidrug resistance genes.
Funder
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Ecology, Evolution, Behavior and Systematics,Microbiology
Reference102 articles.
1. World Health Organization. Antimicrobial resistance: global report on surveillance. World Health Organization. 2014. https://www.who.int/publications/i/item/9789241564748.
2. O’Neill J. Tackling drug-resistant infections globally: final report and recommendations. Government of the United Kingdom. 2016. https://apo.org.au/sites/default/files/resource-files/2016-05/apo-nid63983.pdf.
3. UN Environment. Frontiers 2017: emerging Issues of environmental concern. United Nations Environment Programme. 2017. https://wedocs.unep.org/20.500.11822/22255.
4. Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev. 2010;74:417–33.
5. D’Costa VM, King CE, Kalan L, Morar M, Sung WW, Schwarz C, et al. Antibiotic resistance is ancient. Nature. 2011;477:457–61.
Cited by
54 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献