Abstract
AbstractBone marrow endothelial cells (BMECs) play a key role in bone formation and haematopoiesis. Although recent studies uncovered the cellular taxonomy of stromal compartments in the bone marrow (BM), the complexity of BMECs is not fully characterized. In the present study, using single-cell RNA sequencing, we defined a spatial heterogeneity of BMECs and identified a capillary subtype, termed type S (secondary ossification) endothelial cells (ECs), exclusively existing in the epiphysis. Type S ECs possessed unique phenotypic characteristics in terms of structure, plasticity and gene expression profiles. Genetic experiments showed that type S ECs atypically contributed to the acquisition of bone strength by secreting type I collagen, the most abundant bone matrix component. Moreover, these cells formed a distinct reservoir for haematopoietic stem cells. These findings provide the landscape for the cellular architecture in the BM vasculature and underscore the importance of epiphyseal ECs during bone and haematopoietic development.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
MEXT | Japan Science and Technology Agency
Inamori Foundation
Kao Foundation for Arts and Sciences
Takeda Science Foundation
Mochida Memorial Foundation for Medical and Pharmaceutical Research
Mitsubishi Foundation
Cell Science Research Foundation
SENSHIN Medical Research Foundation
Sumitomo Foundation
Naito Foundation
Uehara Memorial Foundation
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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