Structural basis for recruitment of TASL by SLC15A4 in human endolysosomal TLR signaling
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Published:2023-10-20
Issue:1
Volume:14
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Chen Xudong, Xie Min, Zhang Sensen, Monguió-Tortajada MartaORCID, Yin Jian, Liu Chang, Zhang Youqi, Delacrétaz MaevaORCID, Song Mingyue, Wang YixueORCID, Dong Lin, Ding QiangORCID, Zhou Boda, Tian Xiaolin, Deng Haiteng, Xu Lina, Liu Xiaohui, Yang Zi, Chang QingORCID, Na JieORCID, Zeng WenwenORCID, Superti-Furga GiulioORCID, Rebsamen ManueleORCID, Yang MaojunORCID
Abstract
AbstractToll-like receptors (TLRs) are a class of proteins that play critical roles in recognizing pathogens and initiating innate immune responses. TASL, a recently identified innate immune adaptor protein for endolysosomal TLR7/8/9 signaling, is recruited by the lysosomal proton-coupled amino-acid transporter SLC15A4, and then activates IRF5, which in turn triggers the transcription of type I interferons and cytokines. Here, we report three cryo-electron microscopy (cryo-EM) structures of human SLC15A4 in the apo monomeric and dimeric state and as a TASL-bound complex. The apo forms are in an outward-facing conformation, with the dimeric form showing an extensive interface involving four cholesterol molecules. The structure of the TASL-bound complex reveals an unprecedented interaction mode with solute carriers. During the recruitment of TASL, SLC15A4 undergoes a conformational change from an outward-facing, lysosomal lumen-exposed state to an inward-facing state to form a binding pocket, allowing the N-terminal helix of TASL to be inserted into. Our findings provide insights into the molecular basis of regulatory switch involving a human solute carrier and offers an important framework for structure-guided drug discovery targeting SLC15A4-TASL-related human autoimmune diseases.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
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