Common and divergent gene regulatory networks control injury-induced and developmental neurogenesis in zebrafish retina
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Published:2023-12-20
Issue:1
Volume:14
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Lyu PinORCID, Iribarne MariaORCID, Serjanov Dmitri, Zhai Yijie, Hoang Thanh, Campbell Leah J., Boyd Patrick, Palazzo Isabella, Nagashima Mikiko, Silva Nicholas J.ORCID, Hitchcock Peter F.ORCID, Qian JiangORCID, Hyde David R., Blackshaw SethORCID
Abstract
AbstractFollowing acute retinal damage, zebrafish possess the ability to regenerate all neuronal subtypes through Müller glia (MG) reprogramming and asymmetric cell division that produces a multipotent Müller glia-derived neuronal progenitor cell (MGPC). This raises three key questions. First, do MG reprogram to a developmental retinal progenitor cell (RPC) state? Second, to what extent does regeneration recapitulate retinal development? And finally, does loss of different retinal cell subtypes induce unique MG regeneration responses? We examined these questions by performing single-nuclear and single-cell RNA-Seq and ATAC-Seq in both developing and regenerating retinas. Here we show that injury induces MG to reprogram to a state similar to late-stage RPCs. However, there are major transcriptional differences between MGPCs and RPCs, as well as major transcriptional differences between activated MG and MGPCs when different retinal cell subtypes are damaged. Validation of candidate genes confirmed that loss of different subtypes induces differences in transcription factor gene expression and regeneration outcomes.
Funder
U.S. Department of Health & Human Services | NIH | National Eye Institute
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
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