Derivation of human primordial germ cell-like cells in an embryonic-like culture
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Published:2024-01-02
Issue:1
Volume:15
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Esfahani Sajedeh Nasr, Zheng YiORCID, Arabpour Auriana, Irizarry Agnes M. Resto, Kobayashi Norio, Xue XufengORCID, Shao YueORCID, Zhao Cheng, Agranonik Nicole L., Sparrow Megan, Hunt Timothy J., Faith JaredORCID, Lara Mary Jasmine, Wu Qiu Ya, Silber Sherman, Petropoulos SophieORCID, Yang Ran, Chien Kenneth R.ORCID, Clark Amander T.ORCID, Fu JianpingORCID
Abstract
AbstractPrimordial germ cells (PGCs) are the embryonic precursors of sperm and eggs. They transmit genetic and epigenetic information across generations. Given the prominent role of germline defects in diseases such as infertility, detailed understanding of human PGC (hPGC) development has important implications in reproductive medicine and studying human evolution. Yet, hPGC specification remains an elusive process. Here, we report the induction of hPGC-like cells (hPGCLCs) in a bioengineered human pluripotent stem cell (hPSC) culture that mimics peri-implantation human development. In this culture, amniotic ectoderm-like cells (AMLCs), derived from hPSCs, induce hPGCLC specification from hPSCs through paracrine signaling downstream of ISL1. Our data further show functional roles of NODAL, WNT, and BMP signaling in hPGCLC induction. hPGCLCs are successfully derived from eight non-obstructive azoospermia (NOA) participant-derived hPSC lines using this biomimetic platform, demonstrating its promise for screening applications.
Funder
National Science Foundation
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference38 articles.
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