Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level
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Published:2022-06-24
Issue:1
Volume:13
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Wang Fei, Ding PeiwenORCID, Liang Xue, Ding Xiangning, Brandt Camilla BlunkORCID, Sjöstedt Evelina, Zhu Jiacheng, Bolund Saga, Zhang Lijing, de Rooij Laura P. M. H., Luo Lihua, Wei Yanan, Zhao Wandong, Lv Zhiyuan, Haskó János, Li Runchu, Qin QiuyuORCID, Jia Yi, Wu Wendi, Yuan Yuting, Pu Mingyi, Wang Haoyu, Wu Aiping, Xie Lin, Liu Ping, Chen Fang, Herold Jacqueline, Kalucka JoannaORCID, Karlsson MaxORCID, Zhang Xiuqing, Helmig Rikke Bek, Fagerberg LinnORCID, Lindskog CeciliaORCID, Pontén FredrikORCID, Uhlen MathiasORCID, Bolund Lars, Jessen NielsORCID, Jiang Hui, Xu XunORCID, Yang Huanming, Carmeliet PeterORCID, Mulder JanORCID, Chen Dongsheng, Lin LinORCID, Luo YonglunORCID
Abstract
AbstractPigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-β, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.
Funder
Det Frie Forskningsråd EC | Horizon 2020 Framework Programme Novo Nordisk Fonden Sanming Project of Medicine in Shenzhen
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
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