Bacterial cyclic diguanylate signaling networks sense temperature

Author:

Almblad Henrik,Randall Trevor E.,Liu Fanny,Leblanc KatherineORCID,Groves Ryan A.,Kittichotirat WeerayuthORCID,Winsor Geoffrey L.ORCID,Fournier Nicolas,Au Emily,Groizeleau Julie,Rich Jacquelyn D.,Lou Yuefei,Granton Elise,Jennings Laura K.ORCID,Singletary Larissa A.,Winstone Tara M. L.,Good Nathan M.,Bumgarner Roger E.,Hynes Michael F.,Singh ManuORCID,Stietz Maria Silvina,Brinkman Fiona S. L.,Kumar AyushORCID,Brassinga Ann Karen Cornelia,Parsek Matthew R.,Tseng Boo ShanORCID,Lewis Ian A.ORCID,Yipp Bryan G.ORCID,MacCallum Justin L.,Harrison Joe JonathanORCID

Abstract

AbstractMany bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperature-dependent motility, biofilm development and virulence in the opportunistic pathogenPseudomonas aeruginosa. TdcA synthesizes c-di-GMP with catalytic rates that increase more than a hundred-fold over a ten-degree Celsius change. Analyses using protein chimeras indicate that heat-sensing is mediated by a thermosensitive Per-Arnt-SIM (PAS) domain. TdcA homologs are widespread in sequence databases, and a distantly related, heterologously expressed homolog from the Betaproteobacteria orderGallionellalesalso displayed thermosensitive diguanylate cyclase activity. We propose, therefore, that thermotransduction is a conserved function of c-di-GMP signaling networks, and that thermosensitive catalysis of a second messenger constitutes a mechanism for thermal sensing in bacteria.

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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