RIF1 regulates early replication timing in murine B cells-Reference-Cited by-同舟云学术

RIF1 regulates early replication timing in murine B cells

Published:2023-12-11 Issue:1 Volume:14 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Malzl Daniel,Peycheva Mihaela^ORCID,Rahjouei Ali,Gnan Stefano^ORCID,Klein Kyle N.,Nazarova Mariia^ORCID,Schoeberl Ursula E.^ORCID,Gilbert David M.^ORCID,Buonomo Sara C. B.^ORCID,Di Virgilio Michela^ORCID,Neumann Tobias^ORCID,Pavri Rushad^ORCID

Abstract

AbstractThe mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating murine B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin and promotes early replication, but plays a minor role in regulating replication origin activity, gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal additional layers of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary

Link

https://www.nature.com/articles/s41467-023-43778-y.pdf

Reference100 articles.

1. Marchal, C., Sima, J. & Gilbert, D. M. Control of DNA replication timing in the 3D genome. Nat. Rev. Mol. Cell Biol. 20, 721–737 (2019).

2. Pope, B. D. et al. Topologically associating domains are stable units of replication-timing regulation. Nature 515, 402–405 (2014).

3. Sima, J. et al. Identifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication. Cell 176, 816–830.e18 (2019).

4. Rivera-Mulia, J. C. et al. DNA replication timing alterations identify common markers between distinct progeroid diseases. Proc. Natl. Acad. Sci. USA 114, E10972–E10980 (2017).

5. Platt, E. J., Smith, L. & Thayer, M. J. L1 retrotransposon antisense RNA within ASAR lncRNAs controls chromosome-wide replication timing. J. Cell Biol. 217, 541–553 (2018).