Abstract
AbstractBioprinting of cellular aggregates, such as tissue spheroids, to form three-dimensional (3D) complex-shaped arrangements, has posed a major challenge due to lack of robust, reproducible and practical bioprinting techniques. Here, we demonstrate 3D aspiration-assisted freeform bioprinting of tissue spheroids by precisely positioning them in self-healing yield-stress gels, enabling the self-assembly of spheroids for fabrication of tissues. The presented approach enables the traverse of spheroids directly from the cell media to the gel and freeform positioning of the spheroids on demand. We study the underlying physical mechanism of the approach to elucidate the interactions between the aspirated spheroids and the gel’s yield-stress during the transfer of spheroids from cell media to the gel. We further demonstrate the application of the proposed approach in the realization of various freeform shapes and self-assembly of human mesenchymal stem cell spheroids for the construction of cartilage and bone tissues.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy
Reference48 articles.
1. Dababneh, A. B. & Ozbolat, I. T. Bioprinting technology: a current state-of-the-art review. J. Manuf. Sci. Eng. 136, 061016 (2014).
2. Sun, W. et al. The bioprinting roadmap. Biofabrication 12, 022002 (2020).
3. Leberfinger, A. N. et al. Bioprinting functional tissues. Acta Biomater. 95, 32–49 (2019).
4. Ozbolat, I. T. 3D Bioprinting: Fundamentals, Principles and Applications (Academic Press, London, 2016).
5. Bhattacharjee, T. et al. Writing in the granular gel medium. Sci. Adv. 1, e1500655 (2015).
Cited by
75 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献