A versatile bulk electrotransfection protocol for murine embryonic fibroblasts and iPS cells
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-020-70258-w.pdf
Reference27 articles.
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2. Han, X. et al. CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci. Adv.1, e1500454 (2015).
3. Liu, C., Zhang, L., Liu, H. & Cheng, K. Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications. J. Control. Release266, 17–26 (2017).
4. Gresch, O. et al. New non-viral method for gene transfer into primary cells. Methods33, 151–163 (2004).
5. Weaver, J. C., Smith, K. C., Esser, A. T., Son, R. S. & Gowrishankar, T. A brief overview of electroporation pulse strength–duration space: A region where additional intracellular effects are expected. Bioelectrochemistry87, 236–243 (2012).
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