Author:
Guo Zhenhua,Cai Lijun,Liu Chuanxue,Chen Zhiqiang,Guan Shiwu,Ma Wendong,Pan Guojun
Abstract
AbstractThe sensitivity of rice to low-temperature stress (LTS), especially at the reproductive stage, is a primary factor of rice yield fluctuation in cold cultivate region. Here, the changes of reactive oxygen species (ROS), osmotic adjustment substances, and antioxidants in different tissues were analyzed during rice growing under low temperatures (LT) at the reproductive stage. Results showed that LTS increases the levels of proline (Pro), soluble protein (SP), glutathione (GSH), superoxidase (SOD), and ascorbate peroxidase (APX) in LJ25 (LTS-resistant) and LJ11 (LTS-sensitive). The activities of catalase (CAT) and peroxidase (POD) were significantly increased in LJ25 but decreased in LJ11 under LTS, while an opposite trend in ROS and malondialdehyde (MDA) was observed in both varieties. Moreover, most physicochemical properties were higher in flag leaves and panicles compared with those in leaf sheaths. The expression patterns of OsCOIN, OsCATC, OsMAP1, OsPOX1, and OsAPX were the same with phenotypic changes in Pro and the enzymes encoded by them, confirming the accuracy of the physicochemical analysis. Therefore, only CAT and POD increased more in LJ25, suggesting they could be the key factors used for LT-tolerant breeding of rice in cold regions.
Publisher
Springer Science and Business Media LLC
Reference67 articles.
1. Teixeira, E. I., Fischer, G., Van Velthuizen, H., Walter, C. & Ewert, F. Global hot-spots of heat stress on agricultural crops due to climate change. Agric. For. Meteorol. 170, 206–215 (2013).
2. Xia, N. et al. Effect of cold-water stress at grain filling stage on starch accumulation and related enzyme activities in grains of japonica rice in cold-region. Chin. J. Rice Sci. 30, 62–74 (2016).
3. Sang, T. & Ge, S. Genetics and phylogenetics of rice domestication. Curr. Opin. Genet. Dev. 17, 533–538 (2007).
4. Kovach, M. J., Sweeney, M. T. & McCouch, S. R. New insights into the history of rice domestication. Trends Genet. 23, 578–587 (2007).
5. Guo, Z. et al. Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq. R. Soc. Open Sci. 7, 201081 (2020).