Author:
Hepp Christof,Shiaelis Nicolas,Robb Nicole C.,Vaughan Alison,Matthews Philippa C.,Stoesser Nicole,Crook Derrick,Kapanidis Achillefs N.
Abstract
AbstractThe increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
Funder
Biotechnology and Biological Sciences Research Council
BBSRC studentship
Royal Society
University of Oxford COVID-19 Research Response Fund
Wellcome Trust
Publisher
Springer Science and Business Media LLC
Cited by
17 articles.
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