Author:
Padrez Yaraslau,Golubewa Lena,Kulahava Tatsiana,Vladimirskaja Tatyana,Semenkova Galina,Adzerikho Igor,Yatsevich Olga,Amaegberi Nadezda,Karpicz Renata,Svirko Yuri,Kuzhir Polina,Rutkauskas Danielis
Abstract
AbstractWe demonstrated that wide-field second harmonic generation (SHG) microscopy of lung tissue in combination with quantitative analysis of SHG images is a powerful tool for fast and label-free visualization of the fibrosis pathogenesis in pulmonary arterial hypertension (PAH). Statistical analysis of the SHG images revealed changes of the collagen content and morphology in the lung tissue during the monocrotaline-induced PAH progression in rats. First order statistics disclosed the dependence of the collagen overproduction on time, the second order statistics indicated tightening of collagen fiber network around blood vessels and their spreading into the alveolar region. Fourier analysis revealed that enhancement of the fiber orientation in the collagen network with PAH progression was followed with its subsequent reduction at the terminating phase of the disease. Proposed approach has potential for assessing pulmonary fibrosis in interstitial lung disease, after lung(s) transplantation, cancer, etc.
Funder
Horizon 2020 RISE DiSeTCom
NATO SPS Programme
Academy of Finland via Flagship Programme Photonics Research and Innovation
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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