Author:
Boteanu Raluca M.,Suica Viorel I.,Ivan Luminita,Safciuc Florentina,Uyy Elena,Dragan Emanuel,Croitoru Sorin M.,Grumezescu Valentina,Chiritoiu Marioara,Sima Livia E.,Vlagioiu Constantin,Socol Gabriel,Antohe Felicia
Abstract
Abstract
Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and in a rat tibial defect model (100% viability). Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. The proposed functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury.
Funder
Ministerul Educaţiei Naţionale
Ministerul Educaţiei Naţionale, CNCSIS-UEFISCSU
Publisher
Springer Science and Business Media LLC
Reference48 articles.
1. Balmayor, E. R. et al. Chemically modified RNA induces osteogenesis of stem cells and human tissue explants as well as accelerates bone healing in rats. Biomaterials 87, 131–146 (2016).
2. Hak, D. J. et al. Delayed union and nonunions: Epidemiology, clinical issues, and financial aspects. Injury 45, S3–S7 (2014).
3. Romagnoli, C., Asta, F. D. & Brandi, M. L. Drug delivery using composite scaffolds in the context of bone tissue engineering. Clin. Cases Miner. Bone Metab. 10, 155–161 (2013).
4. Nakamura, T. et al. Recombinant human basic fibroblast growth factor accelerates fracture healing by enhancing callus remodeling in experimental dog tibial fracture. J. Bone Miner. Res. 13, 942–949 (1998).
5. Kawaguchi, H. et al. A local application of recombinant human fibroblast growth factor 2 for tibial shaft fractures: a randomized, placebo-controlled trial. J. Bone Miner. Res. 25, 2459–2467 (2010).
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献