Author:
Hosogai Masahiro,Takeda Masaaki,Maeda Yuyo,Okazaki Takahito,Mitsuhara Takafumi,Ishii Daizo,Shimizu Kiyoharu,Kuwabara Masashi,Yamasaki Fumiyuki,Yuge Louis,Horie Nobutaka
Abstract
AbstractEvaluation of motor function ischemic stroke rat models includes qualitative assessments such as the modified neurological severity score (mNSS). However, mNSS cannot evaluate the function of forelimbs and hindlimbs separately. We quantitatively assessed motor function in a middle cerebral artery occlusion (MCAO) rat model of ischemic stroke. We recorded transcranial stimulation motor evoked potentials (tcMEPs) from MCAO rats and measured the changes in onset latency and amplitude at the forelimbs and hindlimbs up to 28 days after stroke. All MCAO subjects showed hemiparesis. The amplitudes of tcMEPs in both fore- and hindlimbs were inversely correlated with mNSS scores, but the amplitudes in the forelimbs improved later than those in the hindlimbs. The onset latency of tcMEPs in the forelimbs and hindlimbs remained almost unchanged during the follow-up period. Our results showed the differences in tcMEPs amplitude recovery times between the forelimbs and hindlimbs after MCAO, which emphasizes the importance of separately evaluating forelimbs and hindlimbs in post-ischemic stroke models. This minimally invasive and longitudinal quantitative method could be useful for further research on diseases and neurogenesis.
Funder
Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science
Publisher
Springer Science and Business Media LLC
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