Author:
Rosa Ulisses Alves,Ribeiro Geovani de Oliveira,Villanova Fabiola,Luchs Adriana,Milagres Flávio Augusto de Pádua,Komninakis Shirley Vasconcelos,Tahmasebi Roozbeh,Lobato Márcia Cristina Alves Brito Sayão,Brustulin Rafael,Chagas Rogério Togisaki das,Abrão Maria de Fátima Neves dos Santos,Soares Cassia Vitória de Deus Alves,Tinker Rory J,Pandey Ramendra Pati,Raj V. Samuel,Sabino Ester Cerdeira,Deng Xutao,Delwart Eric,Costa Antonio Charlys da,Leal Élcio
Abstract
AbstractDiarrhea remains one of the most common causes of deaths in children. Although many studies have investigated the prevalence of enteric pathogens around the globe some diarrheal episodes remain unexplained. It is possible that some yet-unidentified viral agents could be related to these cases of gastroenteritis. By using viral metagenomics techniques, we screened 251 fecal samples of children between 0.5 to 2.5-year-old with acute diarrhea not associated with common pathogens. These children live in rural areas and have different levels of contact with animals such as pigs, cows and bats. Here we report a complete genome of one mammalian orthoreovirus (MRV) type 3, denoted TO-151/BR, detected in a female child in the state of Tocantins (north of Brazil). Brazilian TO-151/BR strain was classified as MRV-3 based on S1 phylogeny and was closely related to porcine Asian strains. Phylogenetic analyses showed that other segments were more similar to MRV-3s of different geographic locations and hosts, including human and bats, highlighting genome reassortment and lack of host-specific barriers. This is the first report of MRV-3 in South America and a hypothesis of a silent long-term circulation of this virus in Brazil has been raised.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil
Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil
Publisher
Springer Science and Business Media LLC
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