Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation-Reference-Cited by-同舟云学术

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation

Published:2017-11-29 Issue:1 Volume:7 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Remy Séverine,Chenouard Vanessa,Tesson Laurent,Usal Claire,Ménoret Séverine,Brusselle Lucas,Heslan Jean-Marie,Nguyen Tuan Huan,Bellien Jeremy,Merot Jean,De Cian Anne^ORCID,Giovannangeli Carine,Concordet Jean-Paul,Anegon Ignacio

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/s41598-017-16328-y.pdf

Reference34 articles.

1. Geurts, A. M. et al. Knockout rats via embryo microinjection of zinc-finger nucleases. Science 325, 433 (2009).

2. Remy, S. et al. Zinc-finger nucleases: a powerful tool for genetic engineering of animals. Transgenic Res 19, 363–371 (2010).

3. Tesson, L. et al. Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol 29, 695–696 (2011).

4. Remy, S. et al. Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases. Genome Res 24, 1371–1383 (2014).

5. Crispo, M. et al. Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PLoS One 10, e0136690 (2015).

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