Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease

Author:

Santiago Paula Beatriz,da Silva Bentes Kaio Luís,da Silva Waldeyr Mendes Cordeiro,Praça Yanna Reis,Charneau Sébastien,Chaouch Soraya,Grellier Philippe,dos Santos Silva Ferraz Marcos Antônio,Bastos Izabela Marques Dourado,de Santana Jaime Martins,de Araújo Carla Nunes

Abstract

AbstractThe growing interest in microRNAs (miRNAs) over recent years has led to their characterization in numerous organisms. However, there is currently a lack of data available on miRNAs from triatomine bugs (Reduviidae: Triatominae), which are the vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. A comprehensive understanding of the molecular biology of vectors provides new insights into insect-host interactions and insect control approaches, which are key methods to prevent disease incidence in endemic areas. In this work, we describe the miRNome profiles from gut, hemolymph, and salivary gland tissues of the Rhodnius prolixus triatomine. Small RNA sequencing data revealed abundant expression of miRNAs, along with tRNA- and rRNA-derived fragments. Fifty-two mature miRNAs, previously reported in Ecdysozoa, were identified, including 39 ubiquitously expressed in the three tissues. Additionally, 112, 73, and 78 novel miRNAs were predicted in the gut, hemolymph, and salivary glands, respectively. In silico prediction showed that the top eight most highly expressed miRNAs from salivary glands potentially target human blood-expressed genes, suggesting that R. prolixus may modulate the host’s gene expression at the bite site. This study provides the first characterization of miRNAs in a Triatominae species, shedding light on the role of these crucial regulatory molecules.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Financiadora de Estudos e Projetos

Fundação de Apoio à Pesquisa do Distrito Federal

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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