Author:
Zanini Fabio,Che Xibing,Suresh Nina E.,Knutsen Carsten,Klavina Paula,Xie Yike,Domingo-Gonzalez Racquel,Liu Min,Kum Alexander,Jones Robert C.,Quake Stephen R.,Alvira Cristina M.,Cornfield David N.
Abstract
AbstractRapid expansion of the pulmonary microvasculature through angiogenesis drives alveolarization, the final stage of lung development that occurs postnatally and dramatically increases lung gas-exchange surface area. Disruption of pulmonary angiogenesis induces long-term structural and physiologic lung abnormalities, including bronchopulmonary dysplasia, a disease characterized by compromised alveolarization. Although endothelial cells are primary determinants of pulmonary angiogenesis, mesenchymal cells (MC) play a critical and dual role in angiogenesis and alveolarization. Therefore, we performed single cell transcriptomics and in-situ imaging of the developing lung to profile mesenchymal cells during alveolarization and in the context of lung injury. Specific mesenchymal cell subtypes were present at birth with increasing diversity during alveolarization even while expressing a distinct transcriptomic profile from more mature correlates. Hyperoxia arrested the transcriptomic progression of the MC, revealed differential cell subtype vulnerability with pericytes and myofibroblasts most affected, altered cell to cell communication, and led to the emergence of Acta1 expressing cells. These insights hold the promise of targeted treatment for neonatal lung disease, which remains a major cause of infant morbidity and mortality across the world.
Funder
National Heart, Lung, and Blood Institute
Chan Zuckerberg Initiative
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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