Development of a new macrophage-specific TRAP mouse (MacTRAP) and definition of the renal macrophage translational signature

Author:

Hofmeister Andreas,Thomaßen Maximilian C.,Markert Sabrina,Marquardt André,Preußner Mathieu,Rußwurm Martin,Schermuly Ralph T.,Steinhoff Ulrich,Gröne Hermann-Josef,Hoyer Joachim,Humphreys Benjamin D.,Grgic Ivica

Abstract

AbstractTissue macrophages play an important role in organ homeostasis, immunity and the pathogenesis of various inflammation-driven diseases. One major challenge has been to selectively study resident macrophages in highly heterogeneous organs such as kidney. To address this problem, we adopted a Translational Ribosome Affinity Purification (TRAP)- approach and designed a transgene that expresses an eGFP-tagged ribosomal protein (L10a) under the control of the macrophage-specific c-fms promoter to generate c-fms-eGFP-L10a transgenic mice (MacTRAP). Rigorous characterization found no gross abnormalities in MacTRAP mice and confirmed transgene expression across various organs. Immunohistological analyses of MacTRAP kidneys identified eGFP-L10a expressing cells in the tubulointerstitial compartment which stained positive for macrophage marker F4/80. Inflammatory challenge led to robust eGFP-L10a upregulation in kidney, confirming MacTRAP responsiveness in vivo. We successfully extracted macrophage-specific polysomal RNA from MacTRAP kidneys and conducted RNA sequencing followed by bioinformatical analyses, hereby establishing a comprehensive and unique in vivo gene expression and pathway signature of resident renal macrophages. In summary, we created, validated and applied a new, responsive macrophage-specific TRAP mouse line, defining the translational profile of renal macrophages and dendritic cells. This new tool may be of great value for the study of macrophage biology in different organs and various models of injury and disease.

Funder

Philipps-Universität Marburg

Von-Behring-Röntgen-Stiftung

Deutsche Forschungsgemeinschaft

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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