Author:
Tomé-Poderti Lorena,Olivero-Deibe Natalia,Carrión Federico,Portela María Magdalena,Obal Gonzalo,Cabrera Gleysin,Bianchi Sergio,Lima Analia,Addiego Andrés,Durán Rosario,Moratorio Gonzalo,Pritsch Otto
Abstract
AbstractThe Bovine Leukemia Virus (BLV) Envelope (Env) glycoprotein complex is instrumental in viral infectivity and shapes the host’s immune response. This study presents the production and characterization of a soluble furin-mutated BLV Env ectodomain (sBLV-EnvFm) expressed in a stable S2 insect cell line. We purified a 63 kDa soluble protein, corresponding to the monomeric sBLV-EnvFm, which predominantly presented oligomannose and paucimannose N-glycans, with a high content of core fucose structures. Our results demonstrate that our recombinant protein can be recognized from specific antibodies in BLV infected cattle, suggesting its potential as a powerful diagnostic tool. Moreover, the robust humoral immune response it elicited in mice shows its potential contribution to the development of subunit-based vaccines against BLV.
Funder
Agencia Nacional de Investigación e Innovación
Comisión Académica de Posgrado
International Associated Laboratory on Viral Pathogenesis of Leukemia
Fondo para la Convergencia Estructural del Mercosur
Publisher
Springer Science and Business Media LLC