Author:
Wu Diange,Xia Miao,Yan An,Jiang Haotian,Fan Jiaqi,Zhou Siyuan,Wei Xu,Liu Shudong,Chen Baojiang
Abstract
AbstractCarvacrol (CAR) is a plant extract that has been reported to enhance antioxidant activity in animals. However, the effect of CAR on the intestinal health of rabbits is poorly understood. Here, we investigated whether CAR exerts protective effects on the intestinal health of rabbits following lipopolysaccharide (LPS) challenge and whether these effects were mediated via the reduction of intestinal inflammation and the regulation of the intestinal flora. Intestinal damage was assessed in LPS-challenged rabbits treated or not with CAR. The serum levels of inflammatory factors were assessed by enzyme-linked immunosorbent assay. Histopathological changes in the ileum and cecum were examined using hematoxylin and eosin staining. The relative gene expression levels of inflammatory factors and tight junction proteins in the rabbit cecum were determined by qRT-PCR. High-throughput sequencing analysis of the microbial 16S rRNA gene was performed using the Illumina NovaSeq Platform. The results showed that CAR can prevent intestinal inflammation and damage as well as mitigate gut dysbiosis in rabbits following LPS challenge. Our study provides a theoretical reference for the application of dietary CAR in rabbit production.
Funder
Natural Science Foundation of Hebei Province, China
Agricultural Science Park (Base) Project of Hebei Province, China
Modern Agriculture Industry Technology System of Rabbit, China
Publisher
Springer Science and Business Media LLC
Reference66 articles.
1. Yamada, T. et al. Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. Nat. Immunol. 17(6), 687–694 (2016).
2. Peterson, L. W. & Artis, D. Intestinal epithelial cells: Regulators of barrier function and immune homeostasis. Nat. Rev. Immunol. 14(3), 141–153 (2014).
3. Liu, Z. et al. Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway. Int. J. Mol. Med. 41(3), 1560–1572 (2018).
4. Wilms, E. et al. Intestinal barrier function is maintained with aging—A comprehensive study in healthy subjects and irritable bowel syndrome patients. Sci. Rep. 10(1), 475 (2020).
5. Qin, J. et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464(7285), 59–65 (2010).