Author:
Liu Yuguang,Jeraldo Patricio,Herbert William,McDonough Samantha,Eckloff Bruce,de Vera Jean-Pierre,Cockell Charles,Leya Thomas,Baqué Mickael,Jen Jin,Schulze-Makuch Dirk,Walther-Antonio Marina
Abstract
AbstractUnderstanding the impact of long-term exposure of microorganisms to space is critical in understanding how these exposures impact the evolution and adaptation of microbial life under space conditions. In this work we subjected Nostoc sp. CCCryo 231-06, a cyanobacterium capable of living under many different ecological conditions, and also surviving in extreme ones, to a 23-month stay at the International Space Station (the Biology and Mars Experiment, BIOMEX, on the EXPOSE-R2 platform) and returned it to Earth for single-cell genome analysis. We used microfluidic technology and single cell sequencing to identify the changes that occurred in the whole genome of single Nostoc cells. The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions. The cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.
Funder
ERC Advanced Grant “HOME”
European Space Agency
Deutsches Zentrum für Luft- und Raumfahrt
Science and Technology Facilities Council
Deutsche Forschungsgemeinschaft
The Ivan Bowen Family Foundation
National Center for Advancing Translational Science
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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