Author:
Bhaskar Vinay,Saini Sikander,Ansari Shama,Ghai Shubham,Thakur Abhishek,Chopra Suman,Verma Vivekananda,Malakar Dhruba
Abstract
AbstractEndometritis is a uterine inflammatory disease that causes reduced livestock fertility, milk production and lifespan leading to significant economic losses to the dairy industry. Mesenchymal stem cells (MSC) may act as an alternative for inefficacy of antibiotics and rising antibiotic resistance in endometritis. The present study aimed to cure the chronic endometritic buffaloes using allogenic adipose-derived MSCs (AD-MSC). AD-MSCs were isolated from buffalo adipose tissue and characterized by multilineage differentiation as well as MSC-specific markers. The in vivo safety and efficacy were assessed after infusion of AD-MSCs. In safety trial, cells were administered in healthy buffaloes via different routes (IV and IC) followed by examination of clinical and hematological parameters. In efficacy study, AD-MSCs treatments (IV and IC) and antibiotic therapy (ABT) in endometritic buffaloes were comparatively evaluated. AD-MSCs did not induced any immunological reaction in treated buffaloes. PMN count, CRP levels and VDS were significantly (p ≤ 0.05) reduced after AD-MSCs infusions in IV and IC groups and no significant difference was observed in antibiotic group. The IV group was marked with 50% absolute risk reduction in endometritis and 50% live calf births after artificial insemination in comparison with ABT group. Anti-inflammatory cytokines (IL4 and IL10) and anti-microbial peptides (PI3, CATHL4, LCN2 and CST3) expressions were significantly (p ≤ 0.05) upregulated in IV group. The calf delivery rate after the treatments in IV group was higher (50%, 3 calves) than the other groups (IC: 33.3%, 2 calves; ABT: 16.6%, 1 calf). In conclusion, the administration of AD-MSCs through IV route was found to be safe and efficacious for alleviating chronic endometritis in dairy buffaloes.
Publisher
Springer Science and Business Media LLC
Reference70 articles.
1. Sheldon, I. M., Lewis, G. S., LeBlanc, S. & Gilbert, R. O. Defining postpartum uterine disease in cattle. Theriogenology 65, 1516–1530 (2006).
2. Walsh, S. W., Williams, E. J. & Evans, A. C. O. A review of the causes of poor fertility in high milk producing dairy cows. Anim. Reprod. Sci. 123, 127–138 (2011).
3. Pande, M. et al. Endometritis impairs luteal development, function, and nitric oxide and ascorbic acid concentrations in buffalo (Bubalus bubalis). Trop. Anim. Health Prod. 45, 805–810 (2013).
4. Gilbert, R. O. The effects of endometritis on the establishment of pregnancy in cattle. Reprod. Fertil. Dev. 24, 252–257 (2011).
5. Hanafi, E. M., Ahmed, W. M., El-Moez, S. I. A., El-Khadrawy, H. H. & El-Hameed, A. R. A. Effect of clinical endometritis on ovarian activity and oxidative stress status in Egyptian buffalo-cows. Am. J. Agric. Environ. Sci. 4, 530–536 (2008).