Author:
Bao Youwei,Wu Zhaokun,Zhu Xinhua,Wu Jun,Jiang Yinli,Zhang Ying,Zhu Yu,Liu Zheng,Deng Yi,Liu Wenqiang,Wei Mengyi,Luo Weiming,Xiao Yating
Abstract
AbstractThe pathogenesis of allergic asthma is similar to that of allergic rhinitis, with inflammation cells producing and releasing inflammatory mediators and cytokines closely related to CCR3.Based on the theory of "one airway, one disease", the use of CCR3 monoclonal antibody may have a similar effect on allergic rhinitis. However, there are few studies on CCR3 monoclonal antibody in allergic rhinitis. Therefore, the aim of this study was to investigate the effective concentration of CCR3 monoclonal antibody, to compare the effects of different methods of administration, and to examine the lung condition of allergic mice to investigate whether antibody treatment protects the lungs. In this study, we constructed a mouse model of allergic rhinitis and intraperitoneally injected different doses of CCR3 monoclonal antibody (5, 10, and 20 uL/mg) to observe its therapeutic effect: observing changes in tissue morphology of nasal mucosa, infiltration of inflammation, and using ELISA to detect changes in relevant inflammatory mediators and cytokines, studying the role of CCR3 mAb in inhibiting CCR3-related actions on the nasal mucosa of allergic rhinitis mice. Furthermore, In addition, the therapeutic effects of intraperitoneal injection (i.p.) and intranasal administration (i.n.) were studied on the basis of effective concentrations.
Funder
National Natural Science Foundation of China
Jiangxi Provincial Natural Science Foundation of China
Jiangxi Province Clinical Medicine Research Project
Publisher
Springer Science and Business Media LLC
Reference31 articles.
1. Zhou, M. et al. The expression and significance of eotaxin gene in nasal mucosa and CCR3 in bone marrow in model of allergic rhinitis. Lin Chuang Er Bi Yan Hou Ke Za Zhi 20(5), 227–230 (2006).
2. Zhang, Y. et al. CCR3 gene knockout inhibits proliferation, differentiation, and migration of eosinophils in allergic rhinitis model mice. Mol. Immunol. 162, 1–10 (2023).
3. Yuan, J. et al. Gene knockdown of CCR3 reduces eosinophilic inflammation and the Th2 immune response by inhibiting the PI3K/AKT pathway in allergic rhinitis mice. Sci. Rep. 12(1), 5411 (2022).
4. Wu, S. et al. Effects of lentivirus-mediated CCR3 RNA interference on the function of mast cells of allergic rhinitis in mice. Int. Immunopharmacol. 78, 106011 (2020).
5. Zhao, L. et al. Risk factors for asthma in patients with allergic rhinitis in eastern China. Am. J. Otolaryngol. 43(3), 103426 (2022).