Author:
Bisognin Francesco,Ferraro Vincenzo,Sorella Federica,Lombardi Giulia,Lazzarotto Tiziana,Dal Monte Paola
Abstract
AbstractMycobacterium saskatchewanense is a species of pigmented slow-growing Non-Tuberculous Mycobacteria (NTM), positive for Mycobacterium avium complex (MAC) by AccuProbe system. MAC organisms have frequently been isolated from different medical devices. This is the first study reporting isolation of M. saskatchewanense from medical devices and highlights the importance of correctly identifying the NTMs that often colonize sanitary water. GenoType Mycobacterium CM CE-IVD kit (CM) was used as the first step of NTM strain identification, and all positive cultures were found to be components of MAC. Then, GenoType NTM-DR CE-IVD kit (NTM-DR) was used to differentiate the different species. Sub-culture on solid media were used for: (i) phenotypical confirmation by colony morphology and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry; (ii) molecular confirmation by Next Generation Sequencing. All positive cultures were identified as M. intracellulare by CM and NTM-DR assays, whereas colony morphology showed bright yellow scotochromogenic growth. MALDI-TOF analyses identified the strains as M. saskatchewanense with a high score, and identification was confirmed by NGS analysis based on the hsp-65 region. This paper suggests that it is important to actively monitor NTM contamination in medical devices that use sanitary water, to prevent the possibility of patients becoming infected.
Publisher
Springer Science and Business Media LLC
Reference17 articles.
1. Li, T., Abebe, L. S., Cronk, R. & Bartram, J. A systematic review of waterborne infections from nontuberculous mycobacteria in health care facility water systems. Int. J. Hyg. Environ. Health. 220(3), 611–620. https://doi.org/10.1016/j.ijheh.2016.12.002 (2017).
2. Cervia, J. S., Ortolano, G. A. & Canonica, F. P. Hospital tap water: A reservoir of risk for health care-associated infection. Infect. Dis. Clin. Pract. 16(6), 349–353. https://doi.org/10.1097/IPC.0b013e318181fa5e (2008).
3. Donohue, M. J. et al. Increased frequency of nontuberculous mycobacteria detection at potable water taps within the United States. Environ. Sci. Technol. 49(10), 6127–6133. https://doi.org/10.1021/acs.est.5b00496 (2015).
4. Desai, A. N. & Hurtado, R. M. Infections and outbreaks of nontuberculous mycobacteria in hospital settings. Curr. Treat. Options Infect. Dis. 10(2), 169–181. https://doi.org/10.1007/s40506-018-0165-9 (2018).
5. European Centre for Disease Prevention and Control. Invasive cardiovascular infection by Mycobacterium chimaera associated with 3T heater-cooler system used during open-heart surgery – 18 November 2016. Stockholm: ECDC; 2016 Available from: https://www.ecdc.europa.eu/sites/default/files/media/en/publications/Publications/RRA-mycobacterium-chimaera-November-2016.pdf