Author:
Charoenkul Kamonpan,Nasamran Chanakarn,Janetanakit Taveesak,Chaiyawong Supassama,Bunpapong Napawan,Boonyapisitsopa Supanat,Tangwangvivat Ratanaporn,Amonsin Alongkorn
Abstract
AbstractParainfluenza virus type 5 (PIV-5) causes respiratory infection in several animal species and humans. Canine parainfluenza virus type 5 (CPIV-5) causes respiratory disease in domestic dogs worldwide. In this study, we conducted a cross-sectional survey of CPIV-5 in dogs with respiratory symptoms from small animal hospitals in Thailand from November 2015 to December 2018. Our results showed that 32 out of 571 nasal swab samples (5.6%) were positive for CPIV-5 by RT-PCR specific to the NP gene. To characterize the viruses, three representative CPIV-5 were subjected to whole genome sequencing, and an additional ten CPIV-5 were subjected to HN, F, SH and V/P gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai CPIV-5 was closely related to the CPIV-5 isolated from China and Korea. In conclusion, this study constitutes a whole genome characterization of CPIV-5 from dogs in Thailand. The surveillance of CPIV-5 should be further investigated at a larger scale to determine the dynamics, distribution and potential zoonotic transmission of CPIV-5.
Publisher
Springer Science and Business Media LLC
Reference52 articles.
1. Thomas, S. M., Lamb, R. A. & Paterson, R. G. Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54, 891–902 (1988).
2. Chew, F. T., Doraisingham, S., Ling, A. E., Kumarasinghe, G. & Lee, B. W. Seasonal trends of viral respiratory tract infections in the tropics. Epidemiol. Infect. 121. https://doi.org/10.1017/s0950268898008905 (1998).
3. Morgan, O. W. et al. Hospitalization due to human parainfluenza virus-associated lower respiratory tract illness in rural Thailand. Influenza Other Respir. Viruses 7, 280–285. https://doi.org/10.1111/j.1750-2659.2012.00393.x (2013).
4. Ruampunpong, H. et al. Human parainfluenza virus infection in Thai children with lower respiratory tract infection from 2010 to 2013. Southeast Asian J. Trop. Med. Public Health 45, 610–621 (2014).
5. Henrickson, K. J. Parainfluenza viruses. Clin. Microbiol. Rev. 16, 242–264 (2003).
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献