Molecular characterization of root-knot nematodes (Meloidogyne spp.) from Arkansas, USA

Author:

Ye Weimin,Robbins Robert Thomas,Kirkpatrick Terry

Abstract

Abstract Root-knot nematodes (Meloidogyne spp.) are the most common major pathogens of many crops throughout the world, impacting both the quantity and quality of marketable yields. In this study, a total of 244 root-knot nematode populations from various hosts from 39 counties in Arkansas were tested to determine the species diversity. Molecular characterization was performed on these populations by DNA sequencing of the ribosomal DNA 18S-ITS-5.8S, 28S D2/D3 and a mitochondrial DNA fragment flanking cytochrome oxidase gene subunit II - the intergenic spacer. Five species were identified, including M. incognita (Kofoid & White, 1919) Chitwood, 1949 from soybean, cotton, corn and various vegetables (232 samples); M. hapla Chitwood, 1949 from rose (1 sample); M. haplanaria Eisenback, Bernard, Starr, Lee & Tomaszewski, 2003 from okra, tomato, peanut, Indian hawthorn, ash, willow and elm trees (7 samples); M. marylandi Jepson & Golden in Jepson, 1987 from grasses (3 samples); and M. partityla Kleynhans, 1986 from pecan (1 sample) through a combined analysis of DNA sequencing and PCR by species-specific primers. Meloidogyne incognita is the most abundant species that was identified in 95% samples and was the only species in field crops including soybean and cotton, except for one population of M. haplanaria from soybean in Logan County (TK201). Species-specific primers were used to verify M. incognita through PCR by species-specific primers. Unlike historical data, M. arenaria, M. javanica and M. graminis were not detected from any of the samples collected during this study. This result is essential for effective and sustainable management strategies against root-knot nematodes in Arkansas.

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference71 articles.

1. Sasser, J. N. Economic importance of Meloidogyne in tropical countries. In: Lamberti, F. & Taylor, C. E. (eds) Root-Knot Nematodes (Meloidogyne species). Systematics, Biology and Control. London, UK: Academic Press. 359–374 (1979).

2. Hunt, D. & Handoo, Z. A. Taxonomy, identification and principal species. In Root-knot Nematodes. Eds Perry, Moens, and Starr. Publisher: CABI Publishing. 55–97 (2009).

3. Faske, T. R., Overstreet, C., Lawrence, G. & Kirkpatrick, T. L. Important plant parasitic nematodes of row crops in Arkansas, Louisiana, and Mississippi. Pp. 393–431. In S. A. Subbotin and J. J. Chitambar, eds. Plant Parasitic Nematodes In Sustainable Agriculture Of North America. Vol. 2 - Northeastern, Midwestern, and Southern USA. New York: Springer (2018).

4. Kirkpatrick, T. L. & Sullivan, K. Incidence, population density, and distribution of soybean nematodes in Arkansas. Pp. 47–49, In: J. Ross, Ed., Arkansas Soybean Research Studies, 2016. Arkansas Agricultural Experiment Station Research Series 648 (2018).

5. English, L., Popp, J. & Miller, W. Economic contributions of agriculture and food to Arkansas’ gross domestic product, 1997–2012. Arkansas Agricultural Experiment Station Research Report 995, 19 (2014).

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3