Author:
Ramanujam Praveena L.,Mehrotra Sonam,Kumar Ram Parikshan,Verma Shreekant,Deshpande Girish,Mishra Rakesh K.,Galande Sanjeev
Abstract
AbstractSpecial AT-rich binding protein-1 (SATB1) integrates higher-order chromatin architecture with gene regulation, thereby regulating multiple signaling pathways. In mammalian cells SATB1 directly interacts with β-catenin and regulates the expression of Wnt targets by binding to their promoters. Whether SATB1 regulates Wnt/wg signaling by recruitment of β-catenin and/or its interactions with other components remains elusive. Since Wnt/Wg signaling is conserved from invertebrates to humans, we investigated SATB1 functions in regulation of Wnt/Wg signaling by using mammalian cell-lines andDrosophila. Here, we present evidence that in mammalian cells, SATB1 interacts with Dishevelled, an upstream component of the Wnt/Wg pathway. Conversely, ectopic expression of full-length human SATB1 but not that of its N- or C-terminal domains in the eye imaginal discs and salivary glands of third instarDrosophilalarvae increased the expression of Wnt/Wg pathway antagonists and suppressed phenotypes associated with activated Wnt/Wg pathway. These data argue that ectopically-provided SATB1 presumably modulates Wnt/Wg signaling by acting as negative regulator inDrosophila. Interestingly, comparison of SATB1 with PDZ- and homeo-domain containingDrosophilaprotein Defective Proventriculus suggests that both proteins exhibit limited functional similarity in the regulation of Wnt/Wg signaling inDrosophila. Collectively, these findings indicate that regulation of Wnt/Wg pathway by SATB1 is context-dependent.
Funder
Council of Scientific and Industrial Research, India
Department of Biotechnology, Ministry of Science and Technology, India
Scientific and Engineering Research Board
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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