Author:
Malm Magdalena,Saghaleyni Rasool,Lundqvist Magnus,Giudici Marco,Chotteau Veronique,Field Ray,Varley Paul G.,Hatton Diane,Grassi Luigi,Svensson Thomas,Nielsen Jens,Rockberg Johan
Abstract
AbstractThe need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin.
Funder
VINNOVA
Knut och Alice Wallenbergs Stiftelse
Novo Nordisk Fonden
Stiftelsen för Strategisk Forskning
AstraZeneca
Kungliga Tekniska Hogskolan
Publisher
Springer Science and Business Media LLC
Cited by
61 articles.
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