Author:
Li Chenhao,Gao Qingyuan,Jiang Hao,Liu Chengrun,Du Yujun,Li Lisha
Abstract
AbstractImmune cells play an important role in the development of inflammation in type 1 diabetes mellitus, so we want to explore the changes of CD4+T cells and macrophages in vivo, which can provide an experimental basis for immunotherapy based on CD4+T cells and macrophages. The intraperitoneal injection of streptozocin was used to induce a type 1 diabetes mellitus mouse model; the blood glucose, body weight, and the expression of inflammatory factors in the kidney were measured. Immunohistochemistry was applied to determine and analyze the infiltration of CD4+T cells and macrophages in the spleen, pancreas, and kidney. The subtypes of macrophages in the kidney and CD4+T cells in the spleen were analyzed by flow cytometry. Our study suggests that CD4+T cells and macrophages increase, while the inflammatory immune response system is activated in the development of T1DM. CD4+T cells positively correlated with macrophages in the pancreas and kidney of T1DM. CD4+T cells turn to pro-inflammatory subtypes in the spleen of T1DM, while macrophages turn to pro-inflammatory subtypes in the kidney of T1DM. Therefore, regulation of CD4+T cells and macrophages may be a potential target for T1DM and kidney complications.
Funder
Science and Technology Projects of the Education Department of Jilin Province
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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