Author:
Larsson Malin,Rudqvist Nils-Petter,Spetz Johan,Parris Toshima Z.,Langen Britta,Helou Khalil,Forssell-Aronsson Eva
Abstract
Abstract131I is used clinically for therapy, and may be released during nuclear accidents. After the Chernobyl accident papillary thyroid carcinoma incidence increased in children, but not adults. The aims of this study were to compare 131I irradiation-dependent differences in RNA and protein expression in the thyroid and plasma of young and adult rats, and identify potential age-dependent biomarkers for 131I exposure. Twelve young (5 weeks) and twelve adult Sprague Dawley rats (17 weeks) were i.v. injected with 50 kBq 131I (absorbed dose to thyroid = 0.1 Gy), and sixteen unexposed age-matched rats were used as controls. The rats were killed 3–9 months after administration. Microarray analysis was performed using RNA from thyroid samples, while LC–MS/MS analysis was performed on proteins extracted from thyroid tissue and plasma. Canonical pathways, biological functions and upstream regulators were analysed for the identified transcripts and proteins. Distinct age-dependent differences in gene and protein expression were observed. Novel biomarkers for thyroid 131I exposure were identified: (PTH), age-dependent dose response (CA1, FTL1, PVALB (youngsters) and HSPB6 (adults)), thyroid function (Vegfb (adults)). Further validation using clinical samples are needed to explore the role of the identified biomarkers.
Publisher
Springer Science and Business Media LLC
Reference62 articles.
1. IAEA. Chernobyl's legacy: Health, environmental and socio-economic impacts and recommendations to the governments of belarus, the russian federation and ukraine the chernobyl forum International Atomic Energy Agency (IAEA)2005 [INIS-XA-798]. 52]. http://www.iaea.org/NewsCenter/Focus/Chernobyl/pdfs/05-28601_Chernobyl.pdf. http://inis.iaea.org/search/search.aspx?orig_q=RN:36093263. Accessed 30 Nov 2020.
2. UNSCEAR. Evaluation of data on thyroid cancer in regions affected by the chernobyl accident. UNSCEAR Rep. 11–21 (2018).
3. Sinclair, W. K. & Morton, R. A. X-ray and ultraviolet sensitivity of synchronized chinese hamster cells at various stages of the cell cycle. Biophys. J. 5, 1–25 (1965).
4. Hall, E. J., Brown, J. M. & Cavanagh, J. Radiosensitivity and the oxygen effect measured at different phases of the mitotic cycle using synchronously dividing cells of the root meristem of vicia faba. Radiat. Res. 35(3), 622–634 (1968).
5. Cardis, E. & Hatch, M. The chernobyl accident—An epidemiological perspective. Clin. Oncol. (R. Coll. Radiol.). 23(4), 251–260 (2011).
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