Energy Demand of Synaptic Transmission at the Hippocampal Schaffer-Collateral Synapse

Author:

Liotta Agustin1,Rösner Jörg2,Huchzermeyer Christine1,Wojtowicz Anna1,Kann Oliver13,Schmitz Dietmar2456,Heinemann Uwe1,Kovács Richard1

Affiliation:

1. Institute for Neurophysiology, Charité–Universitätsmedizin, Berlin, Germany

2. Neuroscience Research Center, Charité–Universitätsmedizin, Berlin, Germany

3. Institute of Physiology and Pathophysiology, University of Heidelberg, Germany

4. Bernstein Center for Computational Neuroscience Berlin, Berlin, Germany

5. Cluster of Excellence ‘NeuroCure’, Charité-Universitätsmedizin Berlin, Germany

6. DZNE—German Center for Neurodegenerative Diseases, Berlin, Germany.

Abstract

Neuroenergetic models of synaptic transmission predicted that energy demand is highest for action potentials (APs) and postsynaptic ion fluxes, whereas the presynaptic contribution is rather small. Here, we addressed the question of energy consumption at Schaffer-collateral synapses. We monitored stimulus-induced changes in extracellular potassium, sodium, and calcium concentration while recording partial oxygen pressure (pO2) and NAD(P)H fluorescence. Blockade of postsynaptic receptors reduced ion fluxes as well as pO2 and NAD(P)H transients by ~50%. Additional blockade of transmitter release further reduced Na+, K+, and pO2 transients by ~30% without altering presynaptic APs, indicating considerable contribution of Ca2+-removal, transmitter and vesicle turnover to energy consumption.

Publisher

SAGE Publications

Subject

Cardiology and Cardiovascular Medicine,Neurology (clinical),Neurology

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