Tissue clearing and its applications in neuroscience
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Neuroscience
Link
http://www.nature.com/articles/s41583-019-0250-1.pdf
Reference188 articles.
1. Spalteholz, W. Über das Durchsichtigmachen von Menschlichen und Tierischen Präparaten. (S. Hirzel, 1914).
2. Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332–337 (2013). This article is the first to demonstrate in situ synthesis of a hydrogel and its fusion with tissue via covalent bonds to form a cleared tissue–hydrogel hybrid.
3. Yang, B. et al. Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell 158, 945–958 (2014). This article first reports clearing of adult rodents via the vasculature; RNA detection in cleared tissues with single-molecule resolution by single-molecule fluorescence in situ hybridization and that specific formulations of tissue–hydrogel hybrids can significantly expand.
4. Renier, N. et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896–910 (2014).
5. Susaki, E. A. et al. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. Cell 157, 726–739 (2014). This article first reports on the delipidation activity of amino alcohols and the high-performance hydrophilic tissue-clearing method CUBIC and reports tissue clearing of the marmoset brain.
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