SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy-Reference-Cited by-同舟云学术

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy

Published:2015-11-03 Issue:1 Volume:5 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Ball Graeme,Demmerle Justin,Kaufmann Rainer,Davis Ilan,Dobbie Ian M.,Schermelleh Lothar

Abstract

Abstract Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms and will promote routine application of super-resolution SIM imaging in cell biology.

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/srep15915.pdf

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