MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
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Published:2021-11
Issue:11
Volume:18
Page:1294-1303
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ISSN:1548-7091
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Container-title:Nature Methods
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language:en
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Short-container-title:Nat Methods
Author:
Peirsman ArneORCID, Blondeel EvaORCID, Ahmed TasdiqORCID, Anckaert Jasper, Audenaert Dominique, Boterberg Tom, Buzas Krisztina, Carragher NeilORCID, Castellani Gastone, Castro FláviaORCID, Dangles-Marie Virginie, Dawson John, De Tullio Pascal, De Vlieghere EllyORCID, Dedeyne SándorORCID, Depypere Herman, Diosdi AkosORCID, Dmitriev Ruslan I.ORCID, Dolznig HelmutORCID, Fischer SuzanneORCID, Gespach Christian, Goossens Vera, Heino Jyrki, Hendrix AnORCID, Horvath Peter, Kunz-Schughart Leoni A.ORCID, Maes Sebastiaan, Mangodt ChristopheORCID, Mestdagh PieterORCID, Michlíková SoňaORCID, Oliveira Maria JoséORCID, Pampaloni Francesco, Piccinini FilippoORCID, Pinheiro Cláudio, Rahn Jennifer, Robbins Stephen M., Siljamäki Elina, Steigemann PatrickORCID, Sys Gwen, Takayama ShuichiORCID, Tesei AnnaORCID, Tulkens Joeri, Van Waeyenberge Michiel, Vandesompele JoORCID, Wagemans Glenn, Weindorfer Claudia, Yigit NurtenORCID, Zablowsky Nina, Zanoni Michele, Blondeel Phillip, De Wever OlivierORCID
Abstract
AbstractSpheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry,Biotechnology
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37 articles.
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