Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Author:

Sereika MantasORCID,Kirkegaard Rasmus HansenORCID,Karst Søren MichaelORCID,Michaelsen Thomas Yssing,Sørensen Emil AarreORCID,Wollenberg Rasmus Dam,Albertsen MadsORCID

Abstract

AbstractLong-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing.

Funder

Villum Fonden

Grundfos Foundation, grant “Microflora Danica”.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

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