Computational Optimization of Spectral Library Size Improves DIA-MS Proteome Coverage and Applications to 15 Tumors

Author:

Ge Weigang123,Liang Xiao12,Zhang Fangfei12,Hu Yifan3,Xu Luang12,Xiang Nan123,Sun Rui12,Liu Wei123,Xue Zhangzhi12,Yi Xiao123,Sun Yaoting12,Wang Bo4,Zhu Jiang5,Lu Cong5,Zhan Xiaolu6,Chen Lirong7,Wu Yan89,Zheng Zhiguo1011,Gong Wangang1011,Wu Qijun12,Yu Jiekai13,Ye Zhaoming89,Teng Xiaodong4,Huang Shiang5,Zheng Shu13,Liu Tong6,Yuan Chunhui12,Guo Tiannan12ORCID

Affiliation:

1. Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China

2. Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China

3. Westlake Omics (Hangzhou) Biotechnology Co., Ltd., No. 1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou 310024, Zhejiang Province, China

4. Department of Pathology, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310024, Zhejiang Province, China

5. Center for Stem Cell Research and Application, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, Hubei Province, China

6. Harbin Medical University Cancer Hospital, Harbin 150081, Heilongjiang Province, China

7. Department of Pathology, The Second Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China

8. Department of Orthopaedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China

9. Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou 310020, Zhejiang Province, China

10. The Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou 310022, Zhejiang Province, China

11. Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou 310022, Zhejiang Province, China

12. Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang 110000, Liaoning Province, China

13. Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang, China

Funder

Ministry of Science and Technology of the People's Republic of China

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

Westlake Education Foundation

Hangzhou Agriculture and Society Advancement Program

Publisher

American Chemical Society (ACS)

Subject

General Chemistry,Biochemistry

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