Easy, Robust, and Repeatable Online Acid Cleavage of Proteins in Mobile Phase for Fast Quantitative LC-MS Bottom-Up Protein Analysis─Application for Ricin Detection-Reference-Cited by-同舟云学术

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Easy, Robust, and Repeatable Online Acid Cleavage of Proteins in Mobile Phase for Fast Quantitative LC-MS Bottom-Up Protein Analysis─Application for Ricin Detection

Published:2023-08-11 Issue:33 Volume:95 Page:12339-12348
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Naplekov Denis K.¹,Jadeja Siddharth¹,Fučíková Alena Myslivcová²^ORCID,Švec František¹^ORCID,Sklenářová Hana¹^ORCID,Lenčo Juraj¹^ORCID

Affiliation:

1. Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Heyrovského 1203/8, 500 05 Hradec Králové, Czech Republic

2. Department of Biology, Faculty of Science, University of Hradec Králové, Hradecká 1285, 500 03 Hradec Králové, Czech Republic

Funder

European Regional Development Fund

Grantov? Agentura Cesk? Republiky

Grantov? Agentura, Univerzita Karlova

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.3c01772

Reference69 articles.

1. Getting intimate with trypsin, the leading protease in proteomics

2. Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques

3. Protein Digestion: An Overview of the Available Techniques and Recent Developments

4. Bottom-Up Proteomics: Advancements in Sample Preparation

5. High-Throughput Peptide Mass Mapping Using a Microdevice Containing Trypsin Immobilized on a Porous Polymer Monolith Coupled to MALDI TOF and ESI TOF Mass Spectrometers

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