Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2-Reference-Cited by-同舟云学术

Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2

Published:2020-05-22 Issue:13 Volume:92 Page:9399-9404
ISSN:0003-2700
Container-title:Analytical Chemistry
language:en
Short-container-title:Anal. Chem.

Author:

Wang Ji¹,Cai Kun²,Zhang Ruiqing¹³,He Xiaozhou¹,Shen Xinxin¹,Liu Jun¹,Xu Junqiang²,Qiu Feng¹,Lei Wenwen¹,Wang Jinrong¹⁴,Li Xinna¹,Gao Yuan¹⁴,Jiang Yongzhong²,Xu Wenbo¹,Ma Xuejun¹⁵^ORCID

Affiliation:

1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

2. Hubei Center for Disease Control and Prevention, Wuhan 430000, China

3. Hebei General Hospital, Shijiazhuang 050051, China

4. Hebei Medical University, Shijiazhuang 050031, China

5. Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China

Funder

COVID-19 Prevention Research Program of Datong

Key Technologies R&D Program of the National Ministry of Science

China Mega-Projects for Infectious Disease

IVDC

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c01884

Reference21 articles.

1. A new coronavirus associated with human respiratory disease in China

2. WHO Coronavirus disease 2019 (COVID-19) Situation Report-100. https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200429-sitrep-100-covid-19.pdf?sfvrsn=bbfbf3d1_2 (accessed 2020-04-29).

3. WHO WHO Director-General’s opening remarks at the media briefing on COVID-19. https://www.who.int/dg/speeches/detail/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19-9-march-2020. (accessed 2020-03-15).

4. Diagnosing COVID-19: The Disease and Tools for Detection

5. Chest CT for Typical Coronavirus Disease 2019 (COVID-19) Pneumonia: Relationship to Negative RT-PCR Testing

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