Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation

Author:

Allen Joel D.1ORCID,Chawla Himanshi1ORCID,Samsudin Firdaus2,Zuzic Lorena23ORCID,Shivgan Aishwary Tukaram24ORCID,Watanabe Yasunori1ORCID,He Wan-ting567,Callaghan Sean567,Song Ge567,Yong Peter567ORCID,Brouwer Philip J. M.8,Song Yutong910ORCID,Cai Yongfei11,Duyvesteyn Helen M. E.12,Malinauskas Tomas12ORCID,Kint Joeri13,Pino Paco13,Wurm Maria J.13,Frank Martin14ORCID,Chen Bing1115,Stuart David I.1216ORCID,Sanders Rogier W.817,Andrabi Raiees567,Burton Dennis R.56718,Li Sai910ORCID,Bond Peter J.24ORCID,Crispin Max1ORCID

Affiliation:

1. School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, U.K.

2. Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), Singapore 138671

3. Department of Chemistry, Faculty of Science and Engineering, Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, U.K.

4. Department of Biological Sciences, National University of Singapore, Singapore 117543

5. Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California 92037, United States

6. IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California 92037, United States

7. Consortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research Institute, La Jolla, California 92037, United States

8. Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection & Immunity Institute, 1007 MB Amsterdam, The Netherlands

9. Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China

10. Beijing Advanced Innovation Center for Structural Biology and Frontier Research Center for Biological Structure, Beijing 100084, China

11. Division of Molecular Medicine, Boston Children’s Hospital, 3 Blackfan Street, Boston, Massachusetts 02115, United States

12. Division of Structural Biology, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford OX3 7BN, U.K.

13. ExcellGene SA, CH1870 Monthey, Switzerland

14. Biognos AB, Generatorsgatan 1, 41705 Göteborg, Sweden

15. Department of Pediatrics, Harvard Medical School, 3 Blackfan Street, Boston, Massachusetts 02115, United States

16. Diamond Light Source Ltd., Harwell Science & Innovation Campus, Didcot OX11 0DE, U.K.

17. Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10065, United States

18. Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, Massachusetts 02139, United States

Funder

NIH Clinical Center

Medical Research Council

Bill and Melinda Gates Foundation

Wellcome Trust

Vici grant from the Netherlands Organization for Scientific Research

Innovation Fund for Medical Science

University of Southampton Coronavirus Response Fund

BII of A*STAR

Bright Future Trust

Publisher

American Chemical Society (ACS)

Subject

Biochemistry

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