Botulinum Toxin Type A Alleviates Benign Prostatic Hyperplasia by Regulating Progression of Epithelial-Mesenchymal Transition via Inhibition of Hypoxia-Inducible Factor-1α

Author:

Duan Qiuyan1,Li Junhua2,Song Chen2,Gong Xiuqing2,Li Ruipeng2,Zhou Peng2

Affiliation:

1. Department of Dermatology, Hangzhou Third People’s Hospital, No. 38 Xihu Dadao, Shangcheng District, Hangzhou, Zhejiang Province, 310009, China

2. Department of Urology, Hangzhou Third People’s Hospital, No. 38 Xihu Dadao, Shangcheng District, Hangzhou, Zhejiang Province, 310009, China

Abstract

This study proposes to explore the impact of BTX-A on BPD and the functional mechanism. In this study, RWPE-1 cells were stimulated with 10 ng/mL DHT, followed by treatment with 5, 10, and 20 U/mL BTX-A for 24 h. Significantly increased cell viability, repressed apoptosis, upregulated Vimentin, N-cadherin, and FN, and downregulated E-cadherin were observed in DHT-stimulated RWPE-1 cells, which were drastically rescued by BTX-A treatment. A BPH rat model was established, followed by treatment with 20, 60, and 100 U/mL BTX-A. An increased PW/BW ratio, elevated serum DHT levels, severe pathological changes in the prostate tissue, and facilitated EMT progression were observed in BPH rats, which were drastically abolished by BTX-A. Furthermore, in DHT-handled RWPE-1 cells and prostate tissue of BPH rats, HIF-1α and VEGF were significantly upregulated and were greatly downregulated by BTX-A treatment. Finally, the impact of BTX-A on the proliferation and apoptosis of DHT-treated RWPE-1 cells, EMT progression, and VEGF expression was significantly abolished by the overexpression of HIF-1α. Our data revealed that BTX-A alleviated BPH by regulating the progression of EMT via inhibition of HIF-1α.

Publisher

American Scientific Publishers

Subject

Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3