Hsa-Circ-0002360 Promotes the Development of Non-Small Cell Lung Cancer Cells by Regulating Specific E3 Ubiquitin Protein Ligase 2 Pathway Through miRNA-511-5p

Abstract

Lung cancer is one of the most common causes of cancer-related deaths for cancer patients, the specific role of cyclic RNA in non-small cell lung cancer still needs further exploration. The effect of Hsa-Circ-0002360 and the relative pathway participated in the development of non-small cell lung cancer cells, lentivirus expression vector shcirc0002360, miRNA-511-5p mimics and recombinant protein SMURF2 were used in this study. The function of cell proliferation, migration and apoptosis were compared by flow cytometry, wound healing, RT-qPCR and western blot. We found circRNA 0002360 knockout significantly inhibited A549 cell functions. After further overexpression of miR-511-5p, the migration function, proliferation function, and cell viability of cells are significantly decreased while apoptosis rate increased, and quite the opposite after adding SMURF2. Smurf2 promotes the development of non-small cell lung cancer cells by regulating tumor cell migration, dissemination, and apoptosis, and this process is also regulated by Hsa-cic-000236 and miR-511-5p.