Investigation into Biocompatibility and In Vitro Osteogenic Differentiation Ability of New Degradable Polymer Material (Poly-glycerolsebacate-maleic Anhydride) Combined with Nano-Hydroxyapatite Scaffolds

Abstract

This work explored the biocompatibility and In Vitro osteogenic differentiation ability of new degradable polymer scaffold to provide some references for the research focusing on applying the scaffold for osseous tissue engineering. Polyglycerolsebacate-maleic anhydride (PGS-M) and Nano-hyproxyapatite (n-HA) were utilized to prepare a new scaffold, which was called PGS-M/n-HA herein. Besides, scanning electron microscope (SEM) was employed to observe the morphology and porosity of the PGS-M/n-HA, and its In Vitro degradation rate and mechanical property (MP) were analyzed. Human bone marrow mesenchymal stem cells (HBMSCs) (Ctrl group), osteoblast inducing conditional media (OICM group), PGS-M/n-HA group, and PGS-M/n-HA combining with OICM (OICM+PGS-M/n-HA group) were cultured. After that, the differences in cell adhesion rate, viability, and alkaline phosphatase (AKP) activity were compared and analyzed. It was demonstrated that porosities of PGS-M and PGS-M/n-HA declined while the constrained modulus increased. Based on those in the Ctrl group, the cell viability and AKP activity in the OICM group were enhanced. In contrast to those in PGS-M/n-HA group, cell adhesion rate and AKP activity were both enhanced in the OICM+PGS-M/n-HA group. Therefore, it suggested that PGS-M/n-HA scaffold possessed high degradability, MP, and biocompatibility, and could induce the differentiation of HBMSCs into osteoblasts.