Exosomal microRNA-146a-5p from Adipose-Derived Mesenchymal Stem Cells: Preventing H2O2-Induced Oxidative Stress Injury in Human Keratinocyte Cell Line by Modulating SHC SH2 Domain-Binding Protein 1

Abstract

Exosomes secreted by adipose-derived mesenchymal stem cell (ADMSC) may function as novel candidates for wound healing. Here, we isolated the exosomes from ADMSCs and authorized by electron microscope (TEM), nanoparticle tracking analysis, as well as western blotting assay. The effects of ADMSC-derived exosomes on the growth and migration of human keratinocyte cell line (HaCaT) treated with H2O2 were studied. Flow cytometry was applied for measuring cell apoptosis. Migration was evaluated by wound healing and Transwell assays. Reverse transcription-quantitative polymerase chain reaction examined miRNAs and SHC SH2 domain-binding protein 1 (SHCBP1) expression. The integration between miR-146a-5p and SHCBP1 was estimated by dual-luciferase reporter analysis. The results revealed that ADMSC-derived exosomes promoted H2O2-treated HaCaT cell growth, migration, and invasion. miR-146a-5p-silenced ADMSC suppressed the regulation of the exosomes on the biological behaviors of HaCaT cells. SHCBP1 was determined and verified to be a target of miR-146a-5p. Moreover, SHCBP1 inhibition abrogated the miR-146a-5p-mediated cellular processes. In conclusion, ADMSC-derived exosomes carrying miR-146a-5p could protect HaCaT cells from injury by negatively regulating SHCBP1 expression.