Latent Membrane Protein 1 Antibody Inhibited Cell Stemness Growth of Nasopharyngeal Carcinoma by Regulating PI3K/AKT Signaling Pathway
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Published:2020-06-01
Issue:6
Volume:10
Page:768-775
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ISSN:2157-9083
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Container-title:Journal of Biomaterials and Tissue Engineering
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language:en
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Short-container-title:j biomater tissue eng
Author:
Zhang Dawei,Xiong Lin,Li Liang,Chen Yuan,Tang Xiaojun,Tang Qi,Mao Yuan,Ming Hao,Chen Zhi,Du Yinjuan,Wang Yang,Chen Renjie,Zhu Jin
Abstract
Objective: The aim of the study was to investigate the effects of LMP1-Fab antibody on Nasopharyngeal carcinoma (NPC) cancer stem cells (CSCs). Methods/Results: LMP1 was identified to play an important role in maintaining the stemness characteristics of NPC by sphere formation,
as is evidenced by CCK-8 and colony formation assays. Furthermore, CCK8 and TUNEL assays indicated that LMP1-Fab antibody could accelerate cell apoptosis while inhibiting cell proliferation in nasopharyngeal carcinoma stem cell. Notably, LMP1-Fab antibody inhibited NPC xenograft cancer growth
in the nude mice. At the molecular level, LMP1-Fab antibody inhibited the phosphorylation of PI3K/AKT signaling pathway both in vitro and in vivo. More importantly, these effects were blocked by PI3K/AKT phosphorylation inhibitor LY294002. Conclusion: These results suggested
that the novel antibody-targeting LMP1 is likely to be a promising strategy for the treatment of NPC.
Publisher
American Scientific Publishers
Subject
Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology