In Vitro Induction of Epithelialization of Human Gingival Fibroblasts by Mesenchymal Stem Cells Conditioned Medium (MSCCM) and Its Effects on the Secretion of Interleukin-6 and Interleukin-1beta

Abstract

Epithelial injury is difficult to restore normal structure and function upon self-repair. Tissue engineering technology provides hope for epithelial injury. It is showed that human gingival fibroblasts (HGFs) could be ideal seed cells for tissue repair. Our study intends to assess mesenchymal stem cell (MSC) conditioned medium’s effect on HGFs epithelization. HGFs were cultured In Vitro and divided into two groups, including control maintained at complete DMEM medium containing 10% FBS and induction group treated by MSC conditioned medium followed by analysis of cell proliferation by MTT assay, cell apoptosis, Keratin and vimentin expressions by real-time PCR and Western blot. IL-6 and IL-1β secretions by ELISA. MSC conditioned medium induction significantly inhibited HGFs proliferation, increased keratin mRNA and protein levels, reduced vimentin expression, and enhanced IL-6 and IL-1β secretions compared with control (P < 0.05). Caspase 3 activity failed to exhibit statistical significance (P > 0.05). MSC conditioned medium induced HGFs epithelization and promoted IL-6 and IL-1β secretions, thus providing theoretical basis for the repair function of HGFs.