Effect of Propofol Nanoemulsion on Brain Perilymph Metabolism Through Transforming Growth Factor β1/Extracellular Signal Regulated Kinase 5 (TGF-β1/ERK5) Signaling Pathway

Abstract

Propofol takes part in the metabolism of perilymph in the brain. Propofol nanoemulsion can enhance the efficacy of drugs. This study explored how propofol modified by nanoemulsion inhibited the TGF-β1/ERK5 signaling pathway, thus affecting the brain. The role of perilymph metabolism, and its mechanism of action were also clarified. 40 SD rats of clean grade were separated into 4 groups, namely; control group, propofol, propofol nanoemulsion and TGF-β1/ERK5 inhibitor group. We observed the particle size and potential of propofol nanoemulsion, concentration of several groups of immune factors, inflammatory factors, TGF-β1, and ERK5 protein expression. Results from the laser particle size analyzer showed that the average particle size for the propofol nanoemulsion was 87.14 nm. The zeta potential was 0.391 mV, which was close to electrical neutrality. ELISA results showed that the concentrations of IgG, IgA, and lgM in the propofol group, propofol nanoemulsion group, and TGF-β1/ERK5 inhibitor group were evidently lower and the IgG, IgA, IgM concentration for the propofol nanoemulsion group. Moreover, the concentration was lower than that of other groups. ELISA test results showed that the concentrations of IL-12, IL-10, TNF-α, and IL-2 in the propofol group, propofol nanoemulsion group, and TGF-β1/ERK5 inhibitor group were obviously lower. The concentrations of IL-12, IL-10, TNF-α and IL-2 in the propofol nanoemulsion group were lower than those in the other groups (p < 0.05). These results exhibited that, the expression levels of TGF-β1 and ERK5 in the propofol group, propofol nanoemulsion group, and TGF-β1/ERK5 inhibitor group were evidently lower. TGF-β1 and ERK5 expression levels in the propofol nanoemulsion group was lower than in the other groups (p<0.05). Propofol nanoemulsion regulates the TGF-β1/ERK5 signaling pathway, inhibits its expression, reducing inflammation, increasing immune response, and promoting perilymph metabolism in the brain.