Basic Fibroblast Growth Factor (bFGF)-Overexpressed Bone Marrow Mesenchymal Stem Cells (BMSCs) Orchestrate Lung Cancer Development and Fibroblast Growth

Abstract

Lung cancer is one of most common cancer with a complicated pathogenesis and a poor prognosis. This study aimed to investigate the role of bFGF and BMSCs in lung cancer progression. BMSCs were transfected with bFGF mimic or NC and then co-cultured with lung cancer cells followed by measuring cell migration by Transwell assay and proliferation by CCK-8 assay, expression of bFGF, E-cadherin, and Vimentin by RT-qPCR and Western blot. The BMSCs were positive for CD90, CD71, CD29 and CD45. Overexpression of bFGF in BMSCs resulted in increased lung cancer cell proliferation at 24 h, 48 h and 72 h. Meanwhile, bFGF overexpression also significantly promoted cell migration and invasion as well as upregulated bFGF (4.03±0.36 ng/μl) and E-cadherin (3.64±0.27 ng/μl) and downregulated Vimentin (1.45±0.19 ng/μl). In conclusion, co-culture of BMSCs overexpressing bFGF and lung cancer cells enhances BMSCs differentiation and promotes cancer cell development possibly through regulation of E-cadherin and Vimentin expression, indicating that this might be a novel approach for the treatment of lung cancer.